LOCUS       sequence-95060-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: KCNE1_pCSdest
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(383..407)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(461..479)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(462..479)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctacgacc tgtagctctc caggacccgg gcctggacat aggccttgtc cttctcttgc
      121 caggcatcgg actcgatgta gacgttgaat gggtcgttcg agtgctccag cttcttggag
      181 cggatgtagc tcagcatgat gcccagggtg aagaagccga agaatcccag taccatgagg
      241 acgtagaggg cctccagctt gccgtcaccg ctgcgggggg acctgtgggc caggcccgac
      301 atgttgccac cctgctgaac tgtctcctgc cacagcttgg tcagaaaggg cgtcaccgct
      361 gtggtgttag acaggatcat ggtgcctgct tttttgtaca aacttgtgat ccttgaattc
      421 gaatcgatgg gatcctgcaa aaagaacaag tagcttgtat tctatagtgt cacctaaatc
      481 aagcttgctc cgacgtcccc aggcagaatg gcggttccct aaacgagcat tgcttatata
      541 gacctcccat taggcacgcc taccgcccat ttacgtcaat ggaacgccca tttgcgtcat
      601 tgcccctccc cattgacgtc aatggggatg tacttggcag ccatcgcggg ccatttaccg
      661 ccattgacgt caatgggagt actgccaatg taccctggcg tacttccaat agtaatgtac
      721 ttgccaagtt actattaata gatattgatg tactgccaag tgggccattt accgtcattg
      781 acgtcaatag ggggcgtgag aacggatatg aat
//
