LOCUS       sequence-95063-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR9_pCSdest
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(515..539)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(593..611)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(594..611)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaccata attcctgtgt ctgaatctga atcaccttct tcaaaaactg ggggctgagg
      121 ttgcctccac atgctaacgc tgattcaggg cggcagcaga tgctggtgtt ctcagttcct
      181 tcctgcttcc gcttcctgtt caggaacagg gccgcatttt cccatcctct tcggctgtag
      241 gcgtagtcac gtgaccagga gggcggggcg cgcctgccta gcataagctg gaggacggcg
      301 ccctcaccct ggcgggaggt ggatccccag cgcggtgcag ttacatcgca gacatgggct
      361 cgtctatacg ctgctggacg gccggggtct gggctccaca gccggggggc accaccctgg
      421 agctggtgat aggggaggga atcgccgctc gggcgcccgg gcctcgcgcg gagcctgcgt
      481 gcgcgggagt tcacggggca aatcctgccc atggtgcctg cttttttgta caaacttgtg
      541 atccttgaat tcgaatcgat gggatcctgc aaaaagaaca agtagcttgt attctatagt
      601 gtcacctaaa tcaagcttgc tccgacgtcc ccaggcagaa tggcggttcc ctaaacgagc
      661 attgcttata tagacctccc attaggcacg cctaccgccc atttacgtca atggaacgcc
      721 catttgcgtc attg
//
