LOCUS       sequence-95076-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SOD1_pCSdest
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(530..554)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(608..626)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctattggg cgatcccaat tacaccacaa gccaaacgac ttccagcgtt tcctgtcttt
      121 gtactttctt catttccacc tttgcccaag tcatctgctt tttcatggac caccagtgtg
      181 cggccaatga tgcaatggtc tcctgagagt gagatcacag aatcttcaat agacacatcg
      241 gccacaccat ctttgtcagc agtcacattg cccaagtctc caacatgcct ctcttcatcc
      301 tttggcccac cgtgttttct ggatagagga ttaaagtgag gacctgcact ggtacagcct
      361 gctgtattat ctccaaactc atgaacatgg aatccatgca ggccttcagt cagtccttta
      421 atgcttcccc acaccttcac tggtccatta ctttccttct gctcgaaatt gatgatgccc
      481 tgcactgggc cgtcgccctt cagcacgcac acggccttcg tcgccatggt gcctgctttt
      541 ttgtacaaac ttgtgatcct tgaattcgaa tcgatgggat cctgcaaaaa gaacaagtag
      601 cttgtattct atagtgtcac ctaaatcaag cttgctccga cgtccccagg cagaatggcg
      661 gttccctaaa cgagcattgc ttatatagac ctcccattag gcacgcctac cgccca
//
