LOCUS       sequence-95084-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  detection of activity of ubiquitous calpains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Stockholm D, Bartoli M, Sillon G, Bourg N, Davoust J, Richard I
  TITLE     Imaging calpain protease activity by multiphoton FRET in living 
            mice.
  JOURNAL   J Mol Biol. 2005 Feb 11;346(1):215-22. Epub 2004 Dec 16.
  PUBMED    15663939
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2005-02-11"; volume: "346(1)"; pages: "215-22. Epub 
            2004 Dec 16"
COMMENT     Sequence Label: pCMV-calpainsensor
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..710,711..713,714..716))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(42..63)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     391..410
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     650..671
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      713..722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
       61 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      121 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      181 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc
      241 ttctgcttgt cggcggtgat atagacgttg tggctgatgt agttgtactc cagcttgtgc
      301 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      361 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      421 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      481 atgtggtcgg ggtagcggct gaagcactgc acgccccagg tcagggtggt cacgagggtg
      541 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      601 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      661 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
      721 gcgaccggtg gatcccctga gccatccctg ggcatcgcac catacacctc ctgctggccg
      781 cttccggact tgtacagctc gtccatgccg agagtgatcc c
//
