LOCUS       sequence-95108-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty LIC vector; adds an mCherry gene to the C-terminus of your 
            protein of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  TITLE     6-series mammalian LIC expression vectors
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3 mCherry LIC cloning vector (6B)
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             160..867
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(302..320)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     570..589
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(886..904)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(887..904)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(924..941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    930..1041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc ctacttccaa
      121 tccaatattg gtagtgggag caacggcagc agcggatccg tgagcaaggg cgaggaggat
      181 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      241 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      301 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      361 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      421 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      481 gtggtgaccg tgacccagga ctcctccctc caggacggcg agttcatcta caaggtgaag
      541 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      601 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      661 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      721 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      781 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      841 ggcggcatgg acgagctgta caagtagtaa tctagagggc cctattctat agtgtcacct
      901 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
      961 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1021 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattct
//
