LOCUS       sequence-95109-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty LIC vector; adds an mCherry gene to the C-terminus of your 
            protein of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  TITLE     6-series mammalian LIC expression vectors
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3 mCherry LIC cloning vector (6B)
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(14..442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(124..145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     336..355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    488..712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     701..718
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        738..756
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     738..755
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
       61 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
      121 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
      181 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
      241 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
      301 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
      361 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
      421 aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt tctttccgcc
      481 tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc
      541 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
      601 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
      661 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
      721 gatcagcgag ctctagcatt taggtgacac tatagaatag ggccctctag attactactt
      781 gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac
      841 gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc
      901 gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc
      961 gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta
     1021 catc
//
