LOCUS       sequence-95112-e         319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the POT family transporter from Streptococcus thermophilus
            in E. coli as a C-terminally tagged GFP his tagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S
  TITLE     Alternating access mechanism in the POT family of oligopeptide 
            transporters.
  JOURNAL   EMBO J. 2012 Aug 15;31(16):3411-21. doi: 10.1038/emboj.2012.157. 
            Epub 2012 Jun 1.
  PUBMED    22659829
REFERENCE   2  (bases 1 to 319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.157"; journalName: "EMBO J"; date: "2012-08-15- 
            15"; volume: "31"; issue: "16"; pages: "3411-21"
COMMENT     Sequence Label: pWaldo-GFPd_PepTSt
FEATURES             Location/Qualifiers
     source          1..319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(77..97)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tggtggtggt ggtgatgatg atgggacgca agctttttgt agagctcatc catgccatgt
       61 gtaatcccac gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg
      121 atctttcaaa agggcagatt gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc
      181 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacggaac catcttcaat
      241 gctgtggcga attttgaagt tagctttgat tccattcttt tgtttgtctg ccgtgatgta
      301 tacattgtgt gagttatag
//
