LOCUS       sequence-95122-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Venus-tagged human Scribble protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Tatsis N, Lannigan DA, Macara IG
  TITLE     The function of the p190 Rho GTPase-activating protein is controlled
            by its N-terminal GTP binding domain.
  JOURNAL   J Biol Chem. 1998 Dec 18. 273(51):34631-8.
  PUBMED    9852136
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Dec 18. 273(51):34631-8."
COMMENT     Sequence Label: pKvenus-Scribble
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        240..258
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     240..257
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     271..290
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(348..350,351..353,354..866)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(393..414)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(654..673)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg
      121 cggattcccc gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc
      181 ttggcttcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
      241 tttaggtgac actatagaat aacatccact ttgcctttct ctccacaggt gtccactccc
      301 aggtccaact gcacctcggt tctaagcttc tgcaggtcga ctctagaatg gtgagcaagg
      361 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      421 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      481 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      541 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      601 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      661 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      721 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      781 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      841 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccga
//
