LOCUS       sequence-95128-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAD1Z1A
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    66..122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        183..274
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tactggatct atcaacagga gtccaagact agtcgccagg gttttcccag tcacgacgcg
       61 gccgcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat
      121 tcgcgcggcc gcggcctagg tagtagcccg cctaatgagc gggctttttt ttaattcccc
      181 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      241 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
      301 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
      361 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
      421 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
      481 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
      541 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
      601 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
      661 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
      721 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaa
//
