LOCUS       sequence-95131-e         603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAD1Z1A
FEATURES             Location/Qualifiers
     source          1..603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(39..125)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(141..180)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ggctgacatg gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
       61 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      121 atttgatgcc tttaattaag tccaaaaaaa aaggctccaa aaggagcctt taattgtatc
      181 ggtttaccaa tgtttaatca ggctcgcgcc aatttccgca atctgacggt tacgttcatc
      241 catcgtcgcc tggctgccgg tggtataaat caccacaata cggctcggtt taccatccgg
      301 gcccagcgcc gcaataatgc cacggctgcc acgttcgccc gcaccgcttt tatccgcaat
      361 aaaccagcca gccggcagcg cgctacgcag cagcggaccc gccactttat ccgcttccat
      421 ccaatcaatc agttgctggc ggcttgccag ggtcagcagc tcacccgtca gcagtttacg
      481 cagggtggtc gccattgctg ccggcatggt ggtatcacgt tcatcgttcg gaatcgcttc
      541 gttcagttcc ggttcccaac gatccagacg ggtcacatga tcgcccatgt tatgcagaaa
      601 cgc
//
