LOCUS       sequence-95133-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAT1K1A
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    8..64
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        125..216
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggccgcaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
       61 attcgcgcgg ccgcggccta ggtagtagcc cgcctaatga gcgggctttt ttttaattcc
      121 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      181 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg
      241 gcgctgattc cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg
      301 gtgaaagtga aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat
      361 ctgaacagcg gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc
      421 acctttaaag tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa
      481 ctgggccgtc gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa
      541 aaacatctga ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc
      601 gataacaccg cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg
//
