LOCUS       sequence-95136-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAT1K1A
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(68..154)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(170..209)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 caattttgag tgacacagga acacttaacg gctgacatgg ggcgcgccca gctgtctagg
       61 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      121 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ttaattaagt ccaaaaaaaa
      181 aggctccaaa aggagccttt aattgtatcg gtttaccaat gtttaatcag gctcgcgcca
      241 atttccgcaa tctgacggtt acgttcatcc atcgtcgcct ggctgccggt ggtataaatc
      301 accacaatac ggctcggttt accatccggg cccagcgccg caataatgcc acggctgcca
      361 cgttcgcccg caccgctttt atccgcaata aaccagccag ccggcagcgc gctacgcagc
      421 agcggacccg ccactttatc cgcttccatc caatcaatca gttgctggcg gcttgccagg
      481 gtcagcagct cacccgtcag cagtttacgc agggtggtcg ccattgctgc cggcatggtg
      541 gtatcacgtt catcgttcgg aatcgcttcg ttcagttccg gttcccaacg atccagacgg
      601 gtcacatgat cgcccatgtt atgcagaaac gcggtcagtt ctttcggacc gccaatggtc
      661 gtcagcagca ggttcgccgc ggtgttatcg ctcatggtaa tcgccgcgct gcacagttca
      721 cgcacggtca tgccatcggt cagatgtttt tcggtcaccg ggctatattc caccagatcg
      781 ttctggctat aatgaatacg acggcccagt tgttcctggc ccgcatccac acgg
//
