LOCUS       sequence-95143-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses optimized blasticidin S resistance gene (BSD) for C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello
            CC
  TITLE     A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2014 May 30. pii: genetics.114.166389.
  PUBMED    24879462
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 May 30. pii: genetics.114.166389."
COMMENT     Sequence Label: B1979
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac
       61 cgggcccccc ctcgaggtcg acgccctata accggtttca actgaaccta aaatgtcgtg
      121 atcgaccttt cgtcgaatgc atgcccattc aacgttgagc cgatttgagt gctatttcaa
      181 catgaaattt ttgattctgt cctttcttct cgtatcaatt tgctctggag gtggtggatt
      241 cccgattcaa atgttgacaa aatgtggaat ttcaaaagaa accattgatg gcgttcaaag
      301 tgtaatcgac gaacattcgg tatttttaaa acgacttctt agatattgaa aaatttttca
      361 ggcaatgatt aaaattgcta actcgaacaa gcgtgccaaa aaaaccgaaa tcgattcttt
      421 gaaggcagat attgatttat atttagatga acatgcatct gacgacgaca aggtaggatt
      481 tctaatctaa attttacata tttatttttt ccagaaaaga tggattgagt gcttcacaaa
      541 gaacaactcc taattgtctt gatttttcac tttttttttg aataaaaaat tttaacgtgt
      601 tgataagctg taacacttta tattcataac aacaacgtaa caaacgtcat ttaggatcct
      661 ccctcccaca cgtatcccga tggcagcagt tctctgattc ccacagcagt tggttgtcca
      721 tcagaatcct tgacgattgc tttaattcct gggtgcaaat ccaaaagcac ttgtcggcat
      781 cttccacatg gggaaaggat tcctctgttc tcatttccga ttgccacaat gcaagtcaag
      841 tttcctgaga aaaaa
//
