LOCUS       sequence-95148-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAD1K1A
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(64..150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(166..205)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 tttgagtgac acaggaacac ttaacggctg acatggggcg cgcccagctg tctagggcgg
       61 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      121 gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaagtccaa aaaaaaaggc
      181 tccaaaagga gcctttaatt gtatcggttt accaatgttt aatcaggctc gcgccaattt
      241 ccgcaatctg acggttacgt tcatccatcg tcgcctggct gccggtggta taaatcacca
      301 caatacggct cggtttacca tccgggccca gcgccgcaat aatgccacgg ctgccacgtt
      361 cgcccgcacc gcttttatcc gcaataaacc agccagccgg cagcgcgcta cgcagcagcg
      421 gacccgccac tttatccgct tccatccaat caatcagttg ctggcggctt gccagggtca
      481 gcagctcacc cgtcagcagt ttacgcaggg tggtcgccat tgctgccggc atggtggtat
      541 cacgttcatc gttcggaatc gcttcgttca gttccggttc ccaacgatcc agacgggtca
      601 catgatcgcc catgttatgc agaaacgcgg tcagttcttt cggaccgcca atggtcgtca
      661 gcagc
//
