LOCUS       sequence-95211-e        8630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low core fragment expression (low resource allocator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_39
FEATURES             Location/Qualifiers
     source          1..8630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2009..2040
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             2126..2143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2199..2216)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2369..2415
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2471..2859
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2909..3703)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SRLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVIHGDACLPNIMVENGRFSGFNDCGRLGVADRYQDIA
                     LDTRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     3630..3649
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      4109..4328
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             4419..5174
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5177..5427
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             5753..6928
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             6928..7899
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    7972..8445
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     promoter        8569..8603
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
ORIGIN
        1 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg
       61 ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag
      121 catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa
      181 gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag
      241 atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg
      301 acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag
      361 accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca
      421 atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag
      481 cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa
      541 gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg
      601 tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc
      661 attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac
      721 tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg
      781 ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc
      841 attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac
      901 agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt
      961 aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta
     1021 atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc
     1081 ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct
     1141 gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc
     1201 atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg
     1261 gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc
     1321 aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg
     1381 aaaatccacg gtgcaaactg tgcgggtgtc gacaaggttc cgttccctga gcgcatcaag
     1441 ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact
     1501 tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg
     1561 gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc
     1621 tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac
     1681 ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag
     1741 attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag
     1801 aacggaggtt caggtggtgg atccaacgaa aaagaagaac tgaaatccaa aaaagcggaa
     1861 ctgcgcaacc gtatcgaaca gctgaaacag aaacgtgaac aactgaagca gaaaatcgcg
     1921 aacctgcgta aagaaatcga agcttacaaa taatactagt gcggccgctg caggagtcac
     1981 taagggttag ttagttagat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
     2041 aaaacttccc ttggggttat cattggggct cactcaaagg cggtaataac tcatctcaga
     2101 agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt aaacggccgc
     2161 caaaacagcc aagctggaga atgagagaag attttcagcc tgatacagat taaatcagaa
     2221 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     2281 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     2341 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     2401 ggcctttcgt tttatctgtt gtttgtcggt gaactaattc tgattcgcac gggcccatgg
     2461 ctaattccca tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc
     2521 taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa
     2581 gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt
     2641 taagttgctg atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat
     2701 gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt
     2761 aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag
     2821 tacgtactat caacaggttg aactgctgat cttcagatcc tctacgccgg acgcatcgtg
     2881 gccggatctt gcgcaagcgg tctccaattc agaagaactc gtcaagaagg cgatagaagg
     2941 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     3001 cgccgccaag ctcttcagca atatcacggg tatccaacgc tatgtcctga tagcggtccg
     3061 ccacacccag ccggccacag tcgttgaatc cagaaaagcg gccattttcc accatgatat
     3121 tcggcaagca ggcatcgcca tggatcacga cgagatcctc gccgtcgggc atgcgcgcct
     3181 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     3241 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     3301 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     3361 tggatacttt ctcggcagga gcaaggcgag atgacaggag atcctgcccc ggcacttcgc
     3421 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     3481 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     3541 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     3601 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     3661 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     3721 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     3781 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     3841 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     3901 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     3961 tcatcaagct tatgatgtgg tctgtccttt tacagccagt agtgctcgcc gcagtcgagc
     4021 gacagggcga agccctcgag tgagcgagga agcaccaggg aacagcactt atatattctg
     4081 cttacacacg atgcctgaaa aaacttccct tggggttatc cacttatcca cggggatatt
     4141 tttataatta ttttttttat agtttttaga tcttcttttt tagagcgcct tgtaggcctt
     4201 tatccatgct ggttctagag aaggtgttgt gacaaattgc cctttcagtg tgacaaatca
     4261 ccctcaaatg acagtcctgt ctgtgacaaa ttgcccttaa ccctgtgaca aattgccctc
     4321 agaagaagct gttttttcac aaagttatcc ctgcttattg actctttttt atttagtgtg
     4381 acaatctaaa aacttgtcac acttcacatg gatctgtcat ggcggaaaca gcggttatca
     4441 atcacaagaa acgtaaaaat agcccgcgaa tcgtccagtc aaacgacctc actgaggcgg
     4501 catatagtct ctcccgggat caaaaacgta tgctgtatct gttcgttgac cagatcagaa
     4561 aatctgatgg caccctacag gaacatgacg gtatctgcga gatccatgtt gctaaatatg
     4621 ctgaaatatt cggattgacc tctgcggaag ccagtaagga tatacggcag gcattgaaga
     4681 gtttcgcggg gaaggaagtg gttttttatc gccctgaaga ggatgccggc gatgaaaaag
     4741 gctatgaatc ttttccttgg tttatcaaac gtgcgcacag tccatccaga gggctttaca
     4801 gtgtacatat caacccatat ctcattccct tctttatcgg gttacagaac cggtttacgc
     4861 agtttcggct tagtgaaaca aaagaaatca ccaatccgta tgccatgcgt ttatacgaat
     4921 ccctgtgtca gtatcgtaag ccggatggct caggcatcgt ctctctgaaa atcgactgga
     4981 tcatagagcg ttaccagctg cctcaaagtt accagcgtat gcctgacttc cgccgccgct
     5041 tcctgcaggt ctgtgttaat gagatcaaca gcagaactcc aatgcgcctc tcatacattg
     5101 agaaaaagaa aggccgccag acgactcata tcgtattttc cttccgcgat atcacttcca
     5161 tgacgacagg atagtctgag ggttatctgt cacagatttg agggtggttc gtcacatttg
     5221 ttctgaccta ctgagggtaa tttgtcacag ttttgctgtt tccttcagcc tgcatggatt
     5281 ttctcatact ttttgaactg taatttttaa ggaagccaaa tttgagggca gtttgtcaca
     5341 gttgatttcc ttctctttcc cttcgtcatg tgacctgata tcgggggtta gttcgtcatc
     5401 attgatgagg gttgattatc acagtttatt actctgaatt ggctatccgc gtgtgtacct
     5461 ctacctggag tttttcccac ggtggatatt tcttcttgcg ctgagcgtaa gagctatctg
     5521 acagaacagt tcttctttgc ttcctcgcca gttcgctcgc tatgctcggt tacacggctg
     5581 cggcgagcgc tagtgataat aagtgactga ggtatgtgct cttcttatct ccttttgtag
     5641 tgttgctctt attttaaaca actttgcggt tttttgatga ctttgcgatt ttgttgttgc
     5701 tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc aatgattaaa ggatgttcag
     5761 aatgaaactc atggaaacac ttaaccagtg cataaacgct ggtcatgaaa tgacgaaggc
     5821 tatcgccatt gcacagttta atgatgacag cccggaagcg aggaaaataa cccggcgctg
     5881 gagaataggt gaagcagcgg atttagttgg ggtttcttct caggctatca gagatgccga
     5941 gaaagcaggg cgactaccgc acccggatat ggaaattcga ggacgggttg agcaacgtgt
     6001 tggttataca attgaacaaa ttaatcatat gcgtgatgtg tttggtacgc gattgcgacg
     6061 tgctgaagac gtatttccac cggtgatcgg ggttgctgcc cataaaggtg gcgtttacaa
     6121 aacctcagtt tctgttcatc ttgctcagga tctggctctg aaggggctac gtgttttgct
     6181 cgtggaaggt aacgaccccc agggaacagc ctcaatgtat cacggatggg taccagatct
     6241 tcatattcat gcagaagaca ctctcctgcc tttctatctt ggggaaaagg acgatgtcac
     6301 ttatgcaata aagcccactt gctggccggg gcttgacatt attccttcct gtctggctct
     6361 gcaccgtatt gaaactgagt taatgggcaa atttgatgaa ggtaaactgc ccaccgatcc
     6421 acacctgatg ctccgactgg ccattgaaac tgttgctcat gactatgatg tcatagttat
     6481 tgacagcgcg cctaacctgg gtatcggcac gattaatgtc gtatgtgctg ctgatgtgct
     6541 gattgttccc acgcctgctg agttgtttga ctacacctcc gcactgcagt ttttcgatat
     6601 gcttcgtgat ctgctcaaga acgttgatct taaagggttc gagcctgatg tacgtatttt
     6661 gcttaccaaa tacagcaata gtaatggctc tcagtccccg tggatggagg agcaaattcg
     6721 ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt gaaacggatg aagttggtaa
     6781 aggtcagatc cggatgagaa ctgtttttga acaggccatt gatcaacgct cttcaactgg
     6841 tgcctggaga aatgctcttt ctatttggga acctgtctgc aatgaaattt tcgatcgtct
     6901 gattaaacca cgctgggaga ttagataatg aagcgtgcgc ctgttattcc aaaacatacg
     6961 ctcaatactc aaccggttga agatacttcg ttatcgacac cagctgcccc gatggtggat
     7021 tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg ccattacttt gcctgtatgt
     7081 ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg atagtgttga gaagacctct
     7141 cgggtatggt caggtaatga acgtgaccag gagctgctta ctgaggacgc actggatgat
     7201 ctcatccctt cttttctact gactggtcaa cagacaccgg cgttcggtcg aagagtatct
     7261 ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag ctgctgcact taccgaaagt
     7321 gattatcgtg ttctggttgg cgagctggat gatgagcaga tggctgcatt atccagattg
     7381 ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc agcgttatgc aagccgattg
     7441 cagaatgaat ttgctggaaa tatttctgcg ctggctgatg cggaaaatat ttcacgtaag
     7501 attattaccc gctgtatcaa caccgccaaa ttgcctaaat cagttgttgc tcttttttct
     7561 caccccggtg aactatctgc ccggtcaggt gatgcacttc aaaaagcctt tacagataaa
     7621 gaggaattac ttaagcagca ggcatctaac cttcatgagc agaaaaaagc tggggtgata
     7681 tttgaagctg aagaagttat cactctttta acttctgtgc ttaaaacgtc atctgcatca
     7741 agaactagct taagctcacg acatcagttt gctcctggag cgacagtatt gtataagggc
     7801 gataaaatgg tgcttaacct ggacaggtct cgtgttccaa ctgagtgtat agagaaaatt
     7861 gaggccattc ttaaggaact tgaaaagcca gcaccctgat gcgaccacgt tttagtctac
     7921 gtttatctgt ctttacttaa tgtcctttgt tacaggccag aaagcataac tggcctgaat
     7981 attctctctg ggcccactgt tccacttgta tcgtcggtct gataatcaga ctgggaccac
     8041 ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt
     8101 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg ataatcagac
     8161 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc atggtcccac
     8221 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
     8281 ttattagtct ggaaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca
     8341 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacgatccc actcgtgttg
     8401 tcggtctgat tatcggtctg ggaccacggt cccacttgta ttgtcgatca gactatcagc
     8461 gtgagactac gattccatca atgcctgtca agggcaagta ttgacatgtc gtcgtaacct
     8521 gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct gtggattgtt tacggctagc
     8581 tcagtcctag gtactatgct agctactaga gtcagccaag aaagtactag
//
