LOCUS       sequence-95239-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA to mouse Lmo3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Lasek AW, Giorgetti F, Berger KH, Tayor S, Heberlein U
  TITLE     Lmo genes regulate behavioral responses to ethanol in Drosophila 
            melanogaster and the mouse.
  JOURNAL   Alcohol Clin Exp Res. 2011 Sep;35(9):1600-6. doi: 
            10.1111/j.1530-0277.2011.01506.x. Epub 2011 May 20.
  PUBMED    21599714
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1530-0277.2011.01506.x"; journalName: "Alcohol Clin Exp 
            Res"; date: "2011-09"; volume: "35"; issue: "9"; pages: "1600-6"
COMMENT     Sequence Label: pLL3.7 Lmo3.8 shRNA
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..58
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     43..59
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(101..134)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        250..553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cctttgtttc agagaacaaa ggataaggtt agcctttttt ctcgaggtcg acggtatcga
       61 taagctcgct tcacgagatt ccagcaggtc gagggaccta ataacttcgt atagcataca
      121 ttatacgaag ttatattaag ggttccaagc ttaagcggcc gcgtggataa ccgtattacc
      181 gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      241 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      301 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      361 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      421 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      481 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      541 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      601 ctcacgggga tttccaagtc tccaccccat tgacgtcaat ggggagtttg ttttggcacc
      661 aaaatcaacg ggactttcca aaatgtcgta acaacttccg cccccattga cgcaaatggg
      721 gcggg
//
