LOCUS       sequence-95240-e         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA to mouse Lmo3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Lasek AW, Giorgetti F, Berger KH, Tayor S, Heberlein U
  TITLE     Lmo genes regulate behavioral responses to ethanol in Drosophila 
            melanogaster and the mouse.
  JOURNAL   Alcohol Clin Exp Res. 2011 Sep;35(9):1600-6. doi: 
            10.1111/j.1530-0277.2011.01506.x. Epub 2011 May 20.
  PUBMED    21599714
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1530-0277.2011.01506.x"; journalName: "Alcohol Clin Exp 
            Res"; date: "2011-09"; volume: "35"; issue: "9"; pages: "1600-6"
COMMENT     Sequence Label: pLL3.7 Lmo3.5 shRNA
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(178..199)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     527..546
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgta taacttcgta taatgtatgc tatacgaagt tattaggtcc
      121 ctcgacgaat tcctacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      181 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      241 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      301 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      361 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      421 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      481 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      541 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      601 gaagtcgtgc tgcttcatgt ggtcgggg
//
