LOCUS       sequence-95243-e         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector expressing shRNA to mouse Opmr1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Lasek AW, Janak PH, He L, Whistler JL, Heberlein U
  TITLE     Downregulation of mu opioid receptor by RNA interference in the 
            ventral tegmental area reduces ethanol consumption in mice.
  JOURNAL   Genes Brain Behav. 2007 Nov;6(8):728-35. Epub 2007 Mar 26.
  PUBMED    17428267
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Brain
            Behav."; date: "2007-11"; volume: "6(8)"; pages: "728-35. Epub 2007 
            Mar 26"
COMMENT     Sequence Label: pLL3.7 Mor2 shRNA
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(178..199)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgta taacttcgta taatgtatgc tatacgaagt tattaggtcc
      121 ctcgacgaat tcctacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      181 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      241 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      301 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      361 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      421 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      481 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tg
//
