LOCUS       sequence-95244-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  non-targeting shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Lasek AW, Janak PH, He L, Whistler JL, Heberlein U
  TITLE     Downregulation of mu opioid receptor by RNA interference in the 
            ventral tegmental area reduces ethanol consumption in mice.
  JOURNAL   Genes Brain Behav. 2007 Nov;6(8):728-35. Epub 2007 Mar 26.
  PUBMED    17428267
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Brain
            Behav."; date: "2007-11"; volume: "6(8)"; pages: "728-35. Epub 2007 
            Mar 26"
COMMENT     Sequence Label: pLL3.7 Scr shRNA
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..115
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(180..201)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     529..548
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattg tacctagtgg
       61 aaccggaacc cttaaacatg tataacttcg tataatgtat gctatacgaa gttattaggt
      121 ccctcgacga attcctactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      181 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      241 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      301 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      361 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
      421 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      481 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      541 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
      601 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
      661 agggtggtca cgagggtggg ccagg
//
