LOCUS       sequence-95250-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAT7K1A
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    70..126
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        187..278
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcattactgg atctatcaac aggagtccaa gactagtcgc cagggttttc ccagtcacga
       61 cgcggccgca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
      121 gaattcgcgc ggccgcggcc taggtagtag cccgcctaat gagcgggctt ttttttaatt
      181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg
      301 tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc
      361 tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg
      421 atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga
      481 gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac
      541 aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg
      601 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga
      661 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg
      721 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga
      781 acg
//
