LOCUS       sequence-95255-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as 
            cargo..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVAD7K1A
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..67
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        128..219
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcggccgc aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct
       61 cgaattcgcg cggccgcggc ctaggtagta gcccgcctaa tgagcgggct tttttttaat
      121 tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      181 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt
      241 gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc
      301 ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg
      361 gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg
      421 agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa
      481 caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc
      541 gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg
      601 agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc
      661 gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg
      721 aacgaagcga ttccgaacga
//
