LOCUS       sequence-95257-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sensor targeted to N-terminus of Hec1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Welburn JP, Vleugel M, Liu D, Yates JR 3rd, Lampson MA, Fukagawa T, 
            Cheeseman IM
  TITLE     Aurora B phosphorylates spatially distinct targets to differentially
            regulate the kinetochore-microtubule interface.
  JOURNAL   Mol Cell. 2010 May 14;38(3):383-92. doi: 
            10.1016/j.molcel.2010.02.034.
  PUBMED    20471944
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2010.02"; journalName: "Mol Cell"; date: 
            "2010-05-14- 14"; volume: "38"; issue: "3"; pages: "383-92"
COMMENT     Sequence Label: N-term Hec1-targeted Aurora B FRET sensor
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..737
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gccgccacca tggtgagcaa gggcgaggag accacaatgg
       61 gcgtaatcaa gcccgacatg aagatcaagc tgaagatgga gggcaacgtg aatggccacg
      121 ccttcgtgat cgagggcgag ggcgagggca agccctacga cggcaccaac accatcaacc
      181 tggaggtgaa ggagggagcc cccctgccct tctcctacga cattctgacc accgcgttcg
      241 cctacggcaa cagggccttc accaagtacc ccgacgacat ccccaactac ttcaagcagt
      301 ccttccccga gggctactct tgggagcgca ccatgacctt cgaggacaag ggcatcgtga
      361 aggtgaagtc cgacatctcc atggaggagg actccttcat ctacgagata cacctcaagg
      421 gcgagaactt cccccccaac ggccccgtga tgcagaagaa gaccaccggc tgggacgcct
      481 ccaccgagag gatgtacgtg cgcgacggcg tgctgaaggg cgacgtcaag cacaagctgc
      541 tgctggaggg cggcggccac caccgcgttg acttcaagac catctacagg gccaagaagg
      601 cggtgaagct gcccgactat cactttgtgg accaccgcat cgagatcctg aaccacgaca
      661 aggactacaa caaggtgacc gtttacgaga gcgccgtggc ccgcaactcc accgacggca
      721 tggacgagct gtacaaggct ggtaccaagg gtaatggtag g
//
