LOCUS       sequence-95259-e        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of human FOXH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMKO.1-puro/hFOXH1 shRNA1
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             260..685
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(788..810)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(946..966)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
       61 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
      121 gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac
      181 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta
      241 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
      301 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
      361 ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt ccagggtgcc
      421 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
      481 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
      541 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
      601 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      661 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
      721 gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg
      781 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      841 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      901 ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat gtgtgtcagt
      961 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1021 attagtcagc aaccaggtgt ggaaagtccc caggctccc
//
