LOCUS       sequence-95262-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA to mouse Alk.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Lasek AW, Gesch J, Giorgetti F, Kharazia V, Heberlein U
  TITLE     Alk is a transcriptional target of LMO4 and ERalpha that promotes 
            cocaine sensitization and reward.
  JOURNAL   J Neurosci. 2011 Oct 5;31(40):14134-41. doi: 
            10.1523/JNEUROSCI.3415-11.2011.
  PUBMED    21976498
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3415-11"; journalName: "J Neurosci"; date: 
            "2011-10-5- 5"; volume: "31"; issue: "40"; pages: "14134-41"
COMMENT     Sequence Label: pLL3.7 Alk.2 shRNA
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     42..58
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(100..133)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        249..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aacaattcaa gagattgttt gatgctgcgt tgcttttttc tcgaggtcga cggtatcgat
       61 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
      121 tatacgaagt tatattaagg gttccaagct taagcggccg cgtggataac cgtattaccg
      181 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
//
