LOCUS       sequence-95266-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector: contains constitutive mRFP1, compatible as reporter 
            for E. coli/B. subtilis. See pSEVA3b61 for more info..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA3b67Rb
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    153..209
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      252..346
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gaggaagcca tctagtattc accacctttc tctagtaaca tttattgtac aacacgagcc
       61 catttttgtc aaataaaatt taaattatat caacgttaat aagacgttgt caataaaatt
      121 attttgacaa aattcctagg ccgcggccgc gcgaattcga gctcggtacc cggggatcct
      181 ctagagtcga cctgcaggca tgcaagcttg cggccgcgtc gtgactggga aaaccctggc
      241 gactagtctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
      301 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccag gggtccccaa
      361 taattacgat ttaaatcctt caaacttccc aaaggcgagc cctagtgaca ttagaaaacc
      421 gactgtaaaa agtacagtcg gcattatctc atattataaa agccagtcat taggcctatc
      481 tgacaattcc tgaatagagt tcataaacaa tcctgcatga taaccatcac aaacagaatg
      541 atgtacctgt aaagatagcg gtaaatatat tgaattacct ttattaatga attttcctgc
      601 tgtaataatg ggtagaaggt aattactatt attattgata tttaagttaa acccagtaaa
      661 tgaagtccat ggaataatag aaagagaaaa agcattttca ggtataggtg ttttgggaaa
      721 caatttcccc gaaccattat atttctctac atcagaaagg tataaatcat aaaactcttt
      781 gaagtcattc tttacaggag tccaaatacc agagaatgtt ttagatacac catcaaaaat
      841 tgtataaagt ggctctaact tatcccaata acctaactct ccgtcgctat tgtaaccagt
      901 tctaaaagct gtatttgagt ttatcac
//
