LOCUS       sequence-95268-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab2(1-214)
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatggatcc
       61 atgtcatatg cctacctttt caagtacatt atcatcgggg atactggagt aggaaaatcc
      121 tgcttgctcc ttcagtttac cgacaaacgt ttccagccag ttcatgattt gacgattggc
      181 gtcgaattcg gagcccgtat ggtgacaatt gacggaaagc agatcaaact tcaaatttgg
      241 gacacagccg gacaagaatc attccgctcc atcactcgtt cctattatcg tggagccgcc
      301 ggagctcttc tcgtctacga cattacacga cgcgacacat tcaatcattt gacatcttgg
      361 cttgaggatg ccaggcagca cagtaattcc aatatggtta ttatgttgat tggaaataag
      421 agtgacctgg aagcccgtcg cgaagtgaaa cgtgaagagg gagaagcatt cgcacgagag
      481 cacggactcg tattcatgga gacatctgcc aagacggctg ccaacgtgga agaggcgttc
      541 atcgacactg ccaaagagat ctaccgtaag attcaagaag gcgtgttcga cattaacaat
      601 gaggcaaacg gaatcaagtt gggaccacag cactctccaa gctcaccaaa ttctccaggt
      661 ggaaatgcga cgggaggatt gggcggtgga tctggatgct gttaataagt gagtcgacag
      721 atccggctgc taacaaagcc cgaaaggaag ctgagttggg ctgctgccac cgctgagc
//
