LOCUS       sequence-95271-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab2(1-214)
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(682..699)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             712..734
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    749..773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gttagcagcc gggatctgtc gactcactta ttaacagcat ccagatccac cgcccaatcc
       61 tcccgtcgca tttccacctg gagaatttgg tgagcttgga gagtgctgtg gtcccaactt
      121 gattccgttt gcctcattgt taatgtcgaa cacgccttct tgaatcttac ggtagatctc
      181 tttggcagtg tcgatgaacg cctcttccac gttggcagcc gtcttggcag atgtctccat
      241 gaatacgagt ccgtgctctc gtgcgaatgc ttctccctct tcacgtttca cttcgcgacg
      301 ggcttccagg tcactcttat ttccaatcaa cataataacc atattggaat tactgtgctg
      361 cctggcatcc tcaagccaag atgtcaaatg attgaatgtg tcgcgtcgtg taatgtcgta
      421 gacgagaaga gctccggcgg ctccacgata ataggaacga gtgatggagc ggaatgattc
      481 ttgtccggct gtgtcccaaa tttgaagttt gatctgcttt ccgtcaattg tcaccatacg
      541 ggctccgaat tcgacgccaa tcgtcaaatc atgaactggc tggaaacgtt tgtcggtaaa
      601 ctgaaggagc aagcaggatt ttcctactcc agtatccccg atgataatgt acttgaaaag
      661 gtaggcatat gacatggatc catgatgatg atgatgatgg cccatggtat atctccttct
      721 taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag
      781 tgagtcgtat t
//
