LOCUS       sequence-95272-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab5(1-208)
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(751..769)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatggatcc
       61 atggccgccc gaaacgcagg aaccgcccgg cctggaggtc caaacagaac gtgccagttc
      121 aaactcgttc ttctcggaga aagtgctgta ggcaaatcat ctctcgtatt gcgattcgtc
      181 aagggacaat ttcacgagta tcaggagtcg actataggtg ctgcgttcct cactcaaact
      241 gtgtgccttg acgatgcaac aattaaattc gaaatctggg atactgcagg acaagaaaga
      301 tatcattcat tggcaccaat gtactatcga ggagctcaag cggctattgt tgtctacgat
      361 attaccaatc aagaatcttt ccaaaaagcg aaaaattggg taaaagaact gcagcgacag
      421 gcatctccaa atattgtgat ggcattggct ggaaacaagg ctgatgttgc caataagcga
      481 actgttgagt atgaagaagc taatgcttat gctgaggaca atgctctctt gttcatggag
      541 acatctgcga agacctcaat gaacgtgaac gatattttca tggcaatagc taaaaagttg
      601 ccaatcggac cagcacaggg tgaaccaacg ggaactgtcg atatgaacca accacaacag
      661 caacaaaagg gttcatgctg taaataataa gtgactcgac agatccggct gctaacaaag
      721 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taacccctt
//
