LOCUS       sequence-95273-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab5(1-208)
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(660..677)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             690..712
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    727..751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agcagccgga tctgtcgagt cacttattat ttacagcatg aacccttttg ttgctgttgt
       61 ggttggttca tatcgacagt tcccgttggt tcaccctgtg ctggtccgat tggcaacttt
      121 ttagctattg ccatgaaaat atcgttcacg ttcattgagg tcttcgcaga tgtctccatg
      181 aacaagagag cattgtcctc agcataagca ttagcttctt catactcaac agttcgctta
      241 ttggcaacat cagccttgtt tccagccaat gccatcacaa tatttggaga tgcctgtcgc
      301 tgcagttctt ttacccaatt tttcgctttt tggaaagatt cttgattggt aatatcgtag
      361 acaacaatag ccgcttgagc tcctcgatag tacattggtg ccaatgaatg atatctttct
      421 tgtcctgcag tatcccagat ttcgaattta attgttgcat cgtcaaggca cacagtttga
      481 gtgaggaacg cagcacctat agtcgactcc tgatactcgt gaaattgtcc cttgacgaat
      541 cgcaatacga gagatgattt gcctacagca ctttctccga gaagaacgag tttgaactgg
      601 cacgttctgt ttggacctcc aggccgggcg gttcctgcgt ttcgggcggc catggatcca
      661 tgatgatgat gatgatggcc catggtatat ctccttctta aagttaaaca aaattatttc
      721 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgta
//
