LOCUS       sequence-95277-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab10(1-201)
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(659..676)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             689..711
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    726..750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(750..769)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(751..769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaacnntctt cgggcttgtt agcagccgga tctgtcgact cacttactag cagcatcctc
       61 cactgcttct ctgcctctgt ggttgcactg gattcaccgt atcgcgggat tgctcgtcag
      121 tggaatcagg catcttagcc agaattgctt ctgctaaatc gtaaaacgct gtatcgacgt
      181 gaacattcaa tttagctgaa gtctcgtgga agctaatacc atgatcttgt gcgatcttct
      241 ctcctcgttc tctgctcaca actcgtctat cactcatatc acatttattt cccaatatca
      301 ttttaacaac atcttccgat gcatgttcgt caatattgcg caaccacttt gcaatattgt
      361 caaaactttt cgcatttgtg atatcgtaaa ccagcataat tcccattgct ccgcggtaat
      421 atgatgttgt gattgtgtgg aatcgttctt gtccagctgt gtcccagatt tgtaatttaa
      481 tcttttttcc ttttaattcg atagttttaa ttttgaagtc gattccaata gttgagatga
      541 atgtggtgtt aaaggcatca tccgaaaatc tgtacagaat gcaagttttt ccaactcctg
      601 agtcgcctat tagcagcaat ttgaagagca tgtcatacgg tcggcgagcc atggatccat
      661 gatgatgatg atgatggccc atggtatatc tccttcttaa agttaaacaa aattatttct
      721 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggna
      781 tcgagatctc gantcctcna cgccggacgc ancgtggccg gcaatcaacg ggcggacaca
      841 aggtggcggt tgctgggcgc ctatatcgcc gaanatcncc gnaaaggggn aanatcgggg
      901 ctcgnncact tcgggcctca agaanngctt gtttcngcgn ggggntaagg gnggcaaggn
      961 caccgtngnn cnggggggaa ctggttagga gncnatcncn tgggnagann ncnaatcctn
     1021 ggcgganggc ggggcctnaa cggacnntna anccnnnaat nggngnctgg cttccnaang
     1081 cnnggaaggc cacaanaggg gnaaa
//
