LOCUS       sequence-95278-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab10(1-201)
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(637..654)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             667..689
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    704..728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cagccggatc tgtcgactca cttactagca gcatcctcca ctgctactct gcctctgtgg
       61 ttgcactgga ttcaccgtat cgcgggattg ctcgtcagtg gaatcaggca tcttagccag
      121 aattgcttct gctaaatcgt aaaacgctgt atcgacgtga acattcaatt tagctgaagt
      181 ctcgtggaag ctaataccat gatcttgtgc gatcttctct cctcgttctc tgctcacaac
      241 tcgtctatca ctcatatcac atttatttcc caatatcatt ttaacaacat cttccgatgc
      301 atgttcgtca atattgcgca accactttgc aatattgtca aaacttttcg catttgtgat
      361 atcgtaaacc agcataattc ccattgctcc gcggtaatat gatgttgtga ttgtgtggaa
      421 tcgttcttgt ccagctgtgt cccagatttg taatttaatc ttttttcctt ttaattcgat
      481 agttttaatt ttgaagtcga ttccaatagt tgagatgaat gtggtgttaa aggcatcatc
      541 cgaaaatctg tacagaatgc aagtttttcc aactcctgag tcgcctatta gcagcaattt
      601 gaagagcatg tcatacggtc ggcgagccat ggatccatga tgatgatgat gatggcccat
      661 ggtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      721 acaattcccc tatagtgagt cgta
//
