LOCUS       sequence-95283-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab11 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab11(1-211)
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(760..778)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggccatc atcatcatca tcatggatcc
       61 atgggctctc gtgacgatga atacgactac ttgttcaagg ttgttctgat tggagactca
      121 ggcgtcggaa agtcgaatct cctgtctcgt ttcacaagaa atgagttcaa cttggaatca
      181 aaatcaacaa tcggagtcga gtttgccacg agaagcatct cggtagaagg caagacagtg
      241 aaggctcaaa tttgggatac tgctggacag gaacgttacc gtgccatcac atccgcttac
      301 tatcgtgggg ctgtcggagc tctcctagtc tacgacatcg ctaagcatgt gacgtacgag
      361 aatgttgagc gatggttgaa ggagcttcgt gatcacgccg atcagaacat tgtgattatg
      421 ttggtcggaa acaagagcga cttgcgccat ttgcgtgcag ttccaacaga cgaggccaag
      481 atctacgccg aaagaaatca attgtcgttt attgaaacat ctgccctcga cagcaccaac
      541 gttgaagcag ctttcactaa tatcctgacg gaaatctaca aatcagtatc caacaagcat
      601 gtaggaactg acagacaagg atatggcggt ggcagtggta caatcattcc ttcgccagcg
      661 tccgacccac caaagaagca gtgttgcatc ccataataag tgagtcgaca gatccggctg
      721 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      781 aaccccttgg ggcctctaaa cgggtcttga
//
