LOCUS       sequence-95293-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  makes 6x Fusion of Full length ceRab35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Chotard L, Mishra AK, Sylvain MA, Tuck S, Lambright DG, Rocheleau CE
  TITLE     TBC-2 regulates RAB-5/RAB-7-mediated endosomal trafficking in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2010 Jul 1;21(13):2285-96. doi: 
            10.1091/mbc.E09-11-0947. Epub 2010 May 12.
  PUBMED    20462958
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E09-11-0947"; journalName: "Mol Biol Cell"; date: 
            "2010-07-1- 1"; volume: "21"; issue: "13"; pages: "2285-96"
COMMENT     Sequence Label: pET28-ceRab21(1-209)
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(659..676)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             689..711
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcagccggat ctgtcgagtc acttattatc cacatttgca ctttttctga ttggtgcttc
       61 ctgaattgtc cttgagagat acaccgcccg tgcggctctg atcctttgaa gccgactggg
      121 gatttgcgag tttggcggtc agaacgaggc tcgtgatgca tgtgaacatc ggttcgacat
      181 tcttgtcctc tttggcagaa gtctcaaaaa acgagatatt catcgattga gcataattac
      241 gtgcatcact ctccaaaacc actcgacgct cgttttcttc gcatttattg ccgacaagaa
      301 ccttctgaac cgaatcacaa ttattctcaa tctcctgcaa ccaccgtttc acatttccga
      361 aagattctcc atttgtgacg tcatagacga caacgactcc gtgtgttccg cggtagtaag
      421 tcgatgtaat tgtacggaat ctctcttgcc cggcagtatc ccaaatctga agtttgacac
      481 gttgtccatt aatatccatt gtacgaattt taaagtcgac gccgattgtg gtaatgtagt
      541 tttcagagaa ggtgttatct gcaaaacgga gcaacaagct gctctttccg acacccgaat
      601 ctccaataat cagcaatttg aacaaatgat cgtaatcccg agttcccgcc agggatccat
      661 gatgatgatg atgatggccc atggtatatc tccttcttaa agttaaacaa aattattttc
      721 tagagggg
//
