LOCUS       sequence-95298-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains histone modification interacting domain (KDM4A double 
            Tudor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Kungulovski G, Kycia I, Tamas R, Jurkowska RZ, Kudithipudi S, Henry 
            C, Reinhardt R, Labhart P, Jeltsch A
  TITLE     Application of histone modification-specific interaction domains as 
            an alternative to antibodies.
  JOURNAL   Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. 
            Epub 2014 Oct 9.
  PUBMED    25301795
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.170985.113"; journalName: "Genome Res"; date: "2014-11";
            volume: "24"; issue: "11"; pages: "1842-53"
COMMENT     Sequence Label: pGEX-GP-2-KDM4A double Tudor
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(24..29,30..45,46..52)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     65..87
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     710..732
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
       61 tgtgagcgga taacaatttc acacaggaaa cagtattcat gtcccctata ctaggttatt
      121 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
      181 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
      241 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
      301 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
      361 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
      421 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
      481 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
      541 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
      601 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
      661 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
      721 ccacgtttgg tggtggcgac catcctcc
//
