LOCUS       sequence-95309-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains histone modification interacting domain (CBX1 Chromo).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Kungulovski G, Kycia I, Tamas R, Jurkowska RZ, Kudithipudi S, Henry 
            C, Reinhardt R, Labhart P, Jeltsch A
  TITLE     Application of histone modification-specific interaction domains as 
            an alternative to antibodies.
  JOURNAL   Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. 
            Epub 2014 Oct 9.
  PUBMED    25301795
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.170985.113"; journalName: "Genome Res"; date: "2014-11";
            volume: "24"; issue: "11"; pages: "1842-53"
COMMENT     Sequence Label: pGEX-GP-2-CBX1 Chromo
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(46..51,52..67,68..74)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    82..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     87..109
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             121..774
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     732..754
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat
       61 cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagtattc
      121 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt
      181 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa
      241 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat
      301 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac
      361 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg
      421 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt
      481 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa
      541 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat
      601 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa
      661 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca
      721 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
      781 ctgg
//
