LOCUS       sequence-95311-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras with a G15A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras G15A
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..39
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             19..45
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             67..633
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVAKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(645..665)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atggcttcaa gttatccata tgatgtgccc gactatgcct cacttggagg accctctgga
       61 tccaccatga ctgaatataa acttgtggta gttggagctg gtggcgtagc caagagtgcc
      121 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
      181 tcctacagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
      241 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
      301 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
      361 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
      421 gatttgcctt ccagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
      481 attcctttta ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca
      541 ttagttcgag aaattcgaaa acataaagaa aagatgagca aagatggtaa aaagaagaaa
      601 aagaagtcaa agacaaagtg tgtaattatg taagtcgacc ctgtggaatg tgtgtcagtt
      661 agggtgtgga aagtccccag gctccccagc acgcagaagt atgcaaagca tgcatctcaa
      721 ttagtcagca acc
//
