LOCUS       sequence-95314-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras with a S17N mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras S17N
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..44
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             24..50
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             72..638
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKNALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(650..670)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gatcaatggc ttcaagttat ccatatgatg tgcccgacta tgcctcactt ggaggaccct
       61 ctggatccac catgactgaa tataaacttg tggtagttgg agctggcggc gtaggcaaga
      121 acgccttgac gatacagcta attcagaatc attttgtgga cgaatatgat ccaacaatag
      181 aggattccta caggaagcaa gtagtaattg atggagaaac ctgtctcttg gatattctcg
      241 acacagcagg tcaagaggag tacagtgcaa tgagggacca gtacatgagg actggggagg
      301 gctttctttg tgtatttgcc ataaataata ctaaatcatt tgaagatatt caccattata
      361 gagaacaaat taaaagagtt aaggactctg aagatgtacc tatggtccta gtaggaaata
      421 aatgtgattt gccttccaga acagtagaca caaaacaggc tcaggactta gcaagaagtt
      481 atggaattcc ttttattgaa acatcagcaa agacaagaca gggtgttgat gatgccttct
      541 atacattagt tcgagaaatt cgaaaacata aagaaaagat gagcaaagat ggtaaaaaga
      601 agaaaaagaa gtcaaagaca aagtgtgtaa ttatgtaagt cgaccctgtg gaatgtgtgt
      661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttt
//
