LOCUS       sequence-95317-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains BioBrick prefixed and suffixed insert with a modified lac 
            promoter, Shine-Delgarno sequence, 5'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  TITLE     BioBrick(TM) compatible plasmids for Rhodococcus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSRKBB-empty
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    101..122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(137..142,143..160,161..167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     regulatory      270..279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             289..306
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gattcattaa tgcagctggc acgacaggtt tcccgactgg agaattcatc ctgaacttat
       61 ctagacccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcgtctagta gaaggaggag atctggatcc atatgggcgg ttgataagat atcgaaaagc
      241 ttgatggtac cgaggtcgac gacatgcatc catggcggcc gcctcgagca tcaccatcac
      301 catcactgat tctccttacg catctgtgcg gtatttcaca ccgcatgtac tagttaactt
      361 caggacctgc agaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
      421 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
      481 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
      541 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
      601 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atcagtactg
      661 cggcgtcgct gatcgccctc gcgacgttgt gcgggtggct tgtccctgag ggcgctgcga
      721 cagatagcta aaaatctgcg tcaggatc
//
