LOCUS       sequence-95324-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras with G12V and T35S mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras G12V-T35S
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..45
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             25..51
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             join(73..105,106..108,109..639)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPSIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(673..693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gaatcaatgg cttcaagtta tccatatgat gtgcccgact atgcctcact tggaggaccc
       61 tctggatcca ccatgactga atataaactt gtggtagttg gagctgttgg cgtaggcaag
      121 agtgccttga cgatacagct aattcagaat cattttgtgg acgaatatga tccaagcata
      181 gaggattcct acaggaagca agtagtaatt gatggagaaa cctgtctctt ggatattctc
      241 gacacagcag gtcaagagga gtacagtgca atgagggacc agtacatgag gactggggag
      301 ggctttcttt gtgtatttgc cataaataat actaaatcat ttgaagatat tcaccattat
      361 agagaacaaa ttaaaagagt taaggactct gaagatgtac ctatggtcct agtaggaaat
      421 aaatgtgatt tgccttccag aacagtagac acaaaacagg ctcaggactt agcaagaagt
      481 tatggaattc cttttattga aacatcagca aagacaagac agggtgttga tgatgccttc
      541 tatacattag ttcgagaaat tcgaaaacat aaagaaaaga tgagcaaaga tggtaaaaag
      601 aagaaaaaga agtcaaagac aaagtgtgta attatgtaag tcgacggatc cggaattctc
      661 cgtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      841 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat t
//
