LOCUS       sequence-95325-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1022
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     105..122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     106..122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             189..212
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             249..272
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      complement(363..560)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
       61 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      121 gtgaattcga gctcggtacc cggggatccc tcgaggtcga cctgcagggg gaccatggtc
      181 tcagcgcttg gtcgcatcca caatttgaga aaggtggagg ttccggaggt ggatcgggag
      241 gtggatcgtg gagccacccg cagttcgaaa aataataagc ttgatatcga attcctgcag
      301 cccgggggat ccactagttc tagagcggcc gctggatggc ggcgttagta tcgaatcgac
      361 agcagtatag cgaccagcat tcacatacga ttgacgcatg atattacttt ctgcgcactt
      421 aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt
      481 gattaaaata gaacaactac aatataaaaa aactatacaa atgacaagtt cttgaaaaca
      541 agaatctttt tattgtcagt actgattata attggccagt ctttttcaaa taagcattcc
      601 aaccagcatc tctatacctt ttaccttcaa tatctggatc tcttccttta ccaaacaatc
      661 ctctaccaac aatgataata tcagttccag tgctaacaac ttcatcaaca gttctatatt
      721 gttgtcctaa tccatcacct ttatcatcta atccaactcc aggtgtcata ataagccaat
      781 caaatccttc ttcttgtcca cccatatcac gttgggcaat aaatccaata acaaattcct
      841 tatcggattt agcaatttca acagttttt
//
