LOCUS       sequence-95326-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1022
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        75..418
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tgaccatgat tacgccaagc ttgcatgcgc ctgatcgaca gcttgcctcg tccccgccgg
       61 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca
      121 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat
      181 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc
      241 tgcggacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg
      301 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac
      361 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat
      421 gacagtcaac actaagacct atagtgagag agcagaaact catgcctcac cagtagcaca
      481 acgattattt cgattaatgg aactgaagaa aaccaattta tgtgcatcaa ttgatgttga
      541 taccactaag gaattccttg aattaattga taaattgggt ccttatgtat gcttaatcaa
      601 gactcatatt gatataatca atgatttttc ctatgaatcc actattgaac cattattaga
      661 actttcacgt aaacatcaat ttatgatttt tgaagataga aaatttgctg atattggtaa
      721 taccgtgaag aaacaatata ttggtggagt ttataaaatt agtagttggg cagatattac
      781 taatgctcat ggtgtcactg ggaatggagt agttgaagga ttaaaacagg gagctaaaga
      841 aaccaccacc aaccaagagc caagagggtt attgatgtta gctgaattat catcagtggg
      901 atcattagca tatggagaat attctcaaaa aactgttgaa attgctaaat ccgataagga
      961 atttgttatt ggatttattg cccaacgtga tatgggtgga caagaagaag gatttgattg
     1021 gcttattatg acacctggag ttggattaga tgataaaggt gatggattag gacaac
//
