LOCUS       sequence-95331-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TFF3_pCSdest
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(291..315)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(369..387)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(370..387)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gtaatacgac tcactatagt tctagaggct cgagaggatc accactttgt acaagaaagc
       61 tgggttctag aaggtgcatt ctgcttcctg caggggcttg aaacaccaag gcactccagg
      121 gatcctggag tcaaagcagc agccccggtt gttgcactcc ttgggggtga catgggggta
      181 gccgcagtcc accctgtcct tggctggcac ggcacactgg tttgcagaca ggcccacgta
      241 ctcctcagca gagctggagg acagcaaggc caggaccagc cccagcatgg tgcctgcttt
      301 tttgtacaaa cttgtgatcc ttgaattcga atcgatggga tcctgcaaaa agaacaagta
      361 gcttgtattc tatagtgtca cctaaatcaa gcttgctccg acgtccccag gcagaatggc
      421 ggttccctaa acgagcattg cttatataga cctcccatta ggcacgccta ccgcccattt
      481 acgtcaatgg aacgcccatt tgcgtcattg cccctcccca ttgacgtcaa tggggatgta
      541 cttggcagcc atcgcgggcc atttaccgcc attgacgtca atgggagtac tgccaatgta
      601 ccctggcgta cttccaatag taatgtactt gccaagttac tattaataga tattgatgta
      661 ctgccaagtg ggccatttac cgtcattgac gtcaataggg ggcgtgagaa cggatatgaa
      721 tgggcaatga gccatcccat tgacgtcaat ggtgggtggt cctattgacg tcaatgggca
      781 ttgagccagg cgggccattt accgtaattg acgtcaatg
//
