LOCUS       sequence-95345-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: BRWD1_pCSdest
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(428..452)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(506..524)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(507..524)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaaatta aggtaccttt tgctgtacgt agcaaagact gccttcctgc accaagtaaa
      121 gaagtgactc ttgaaatact gggtggaatt tctttatcca acataggacc gatgcgctgg
      181 cagatttgca aaagatgatc aggagccaca tgcttattgg acaagaccaa ctcctcgtag
      241 ctcctgttgt gctcgttgcc ctcccagtcc aatctcttcg gcaacaactg gtactgctcc
      301 agctcctgca ccagcacctg ggccgctctc cgacacgggc ccgccgatag gtaccgggcg
      361 ataaggaagt acagctccga ctcgatgaga ggcaccgggc gtcgggcgga cgacggctcc
      421 gccatggtgc ctgctttttt gtacaaactt gtgatccttg aattcgaatc gatgggatcc
      481 tgcaaaaaga acaagtagct tgtattctat agtgtcacct aaatcaagct tgctccgacg
      541 tccccaggca gaatggcggt tccctaaacg agcattgctt atatagacct cccattaggc
      601 acgcctaccg cccatttacg tcaatggaac gcccatttgc gtcattgccc ctccccattg
      661 acgtcaatgg ggatgtactt ggcagccatc gcgggccatt taccgccatt gacgtcaatg
      721 ggagtactgc caatgtaccc tggcgtactt ccaatagtaa tgtacttgcc aagttactat
      781 taatagatat tgatgtactg ccaagtgggc catttaccgt cattgacgtc aata
//
