LOCUS       sequence-95355-e        2085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  integrin a5 wild-type headpiece expression (a5 beta-propeller and a5
            thigh domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2085)
  TITLE     integrin a5b1 headpiece
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1/hpa5
FEATURES             Location/Qualifiers
     source          1..2085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1942..1962
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2026..2037
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2062..2085
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atggggagcc ggacgccaga gtcccctctc cacgccgtgc agctgcgctg gggcccccgg
       61 cgccgacccc cgctgctgcc gctgctgttg ctgctgctgc cgccgccacc cagggtcggg
      121 ggcttcaact tagacgcgga ggccccagca gtactctcgg ggcccccggg ctccttcttc
      181 ggattctcag tggagtttta ccggccggga acagacgggg tcagtgtgct ggtgggagca
      241 cccaaggcta ataccagcca gccaggagtg ctgcagggtg gtgctgtcta cctctgtcct
      301 tggggtgcca gccccacaca gtgcaccccc attgaatttg acagcaaagg ctctcggctc
      361 ctggagtcct cactgtccag ctcagaggga gaggagcctg tggagtacaa gtccttgcag
      421 tggttcgggg caacagttcg agcccatggc tcctccatct tggcatgcgc tccactgtac
      481 agctggcgca cagagaagga gccactgagc gaccccgtgg gcacctgcta cctctccaca
      541 gataacttca cccgaattct ggagtatgca ccctgccgct cagatttcag ctgggcagca
      601 ggacagggtt actgccaagg aggcttcagt gccgagttca ccaagactgg ccgtgtggtt
      661 ttaggtggac caggaagcta tttctggcaa ggccagatcc tgtctgccac tcaggagcag
      721 attgcagaat cttattaccc cgagtacctg atcaacctgg ttcaggggca gctgcagact
      781 cgccaggcca gttccatcta tgatgacagc tacctaggat actctgtggc tgttggtgaa
      841 ttcagtggtg atgacacaga agactttgtt gctggtgtgc ccaaagggaa cctcacttac
      901 ggctatgtca ccatccttaa tggctcagac attcgatccc tctacaactt ctcaggggaa
      961 cagatggcct cctactttgg ctatgcagtg gccgccacag acgtcaatgg ggacgggctg
     1021 gatgacttgc tggtgggggc acccctgctc atggatcgga cccctgacgg gcggcctcag
     1081 gaggtgggca gggtctacgt ctacctgcag cacccagccg gcatagagcc cacgcccacc
     1141 cttaccctca ctggccatga tgagtttggc cgatttggca gctccttgac ccccctgggg
     1201 gacctggacc aggatggcta caatgatgtg gccatcgggg ctccctttgg tggggagacc
     1261 cagcagggag tagtgtttgt atttcctggg ggcccaggag ggctgggctc taagccttcc
     1321 caggttctgc agcccctgtg ggcagccagc cacaccccag acttctttgg ctctgccctt
     1381 cgaggaggcc gagacctgga tggcaatgga tatcctgatc tgattgtggg gtcctttggt
     1441 gtggacaagg ctgtggtata caggggccgc cccatcgtgt ccgctagtgc ctccctcacc
     1501 atcttccccg ccatgttcaa cccagaggag cggagctgca gcttagaggg gaaccctgtg
     1561 gcctgcatca accttagctt ctgcctcaat gcttctggaa aacacgttgc tgactccatt
     1621 ggtttcacag tggaacttca gctggactgg cagaagcaga agggaggggt acggcgggca
     1681 ctgttcctgg cctccaggca ggcaaccctg acccagaccc tgctcatcca gaatggggct
     1741 cgagaggatt gcagagagat gaagatctac ctcaggaacg agtcagaatt tcgagacaaa
     1801 ctctcgccga ttcacatcgc tctcaacttc tccttggacc cccaagcccc agtggacagc
     1861 cacggcctca ggccagccct acattatcag agcaagagcc ggatagagga caaggctcag
     1921 atcttgctga ccggtggact ggagaatctt tatttccaag gtggagaaaa tgctcagtgc
     1981 gaaaaagagc tccaggcact tgagaaggaa aatgcacagc tggaatggga attacaagca
     2041 ctagagaagg agctagctca atggagccac ccccagttcg aaaag
//
