LOCUS       sequence-95360-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: S100B_pCSdest
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      313..322
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(344..368)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(422..440)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(423..440)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctactcat gttcaaagaa ctcgtggcag gcagtagtaa ccatggcaac aaaggccatg
      121 aattcctgga agtcacattc gccgtctcca tcattgtcca gtgtttccat gactttgtcc
      181 acaacctcct gctctttgat ttcctctaag aaatgggaaa gctcattgtt gatgagctcc
      241 ttgagttcgg atttcttcag cttgtgcttg tctccctccc ttccagaata ttggtggaaa
      301 acgtcgatga gggccaccat ggccttctcc agctcagaca tggtgcctgc ttttttgtac
      361 aaacttgtga tccttgaatt cgaatcgatg ggatcctgca aaaagaacaa gtagcttgta
      421 ttctatagtg tcacctaaat caagcttgct ccgacgtccc caggcagaat ggcggttccc
      481 taaacgagca ttgcttatat agacctccca ttaggcacgc ctaccgccca tttacgtcaa
      541 tggaacgccc atttgcgtca ttgcccctcc ccattgacgt caatggggat gtacttggca
      601 gccatcgcgg gccatttacc gccattgacg tcaatgggag tactgccaat gtaccctggc
      661 gtacttccaa tagtaatgta cttgccaagt tactattaat agatattgat gtactgccaa
      721 gtgggccatt taccgtcatt gacgtcaata gggggcgtga gaacggatat gaatgggcaa
      781 tgagccatcc cattgacgtc aat
//
