LOCUS       sequence-95361-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: HMGN1_pCSdest
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(368..392)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(446..464)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(447..464)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaatcag acttggcttc tttctctcct gcttcatcag aggctggact ctcctcagtc
      121 ttcgtttccc cgttttccgc aggtaagtct tctttagttt cttggttagc cacttcggcc
      181 tgttttccct ttgctcccct tttccctttt gtttgcactt ttttgtctga agatttatcc
      241 ttcgctgctg cctttttcgg cttcgcttcc acttttgcag gaggtttagc tgacaaccgc
      301 gccgatctcc tcttgggctc ttccttggcg gcgccttcgg cggagctgac cttcctcttg
      361 ggcatggtgc ctgctttttt gtacaaactt gtgatccttg aattcgaatc gatgggatcc
      421 tgcaaaaaga acaagtagct tgtattctat agtgtcacct aaatcaagct tgctccgacg
      481 tccccaggca gaatggcggt tccctaaacg agcattgctt atatagacct cccattaggc
      541 acgcctaccg cccatttacg tcaatggaac gcccatttgc gtcattgccc ctccccattg
      601 acgtcaatgg ggatgtactt ggcagccatc gcgggccatt taccgccatt gacgtcaatg
      661 ggagtactgc caatgtaccc tggcgtactt ccaatagtaa tgtacttgcc aagttactat
      721 taatagatat tgatgtactg ccaagtgggc catttaccgt cattgacgtc aatagggggc
      781 gtgagaacgg atatgaatgg gcaatgagcc atcccattga cgtcaat
//
