LOCUS       sequence-95363-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1021
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             278..301
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             338..361
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(397..413)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(397..413)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc ggggatccct
      241 cgaggtcgac ctgcaggggg accatggtct cagcgcttgg tcgcatccac aatttgagaa
      301 aggtggaggt tccggaggtg gatcgggagg tggatcgtgg agccacccgc agttcgaaaa
      361 ataataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct agagcggccg
      421 cggaacgatc attcactata tatatatcaa tttatatata cgtatgtgta attgaagaaa
      481 gatacgtttt ttcctctatt gagaggcctg ctggatgaat agctttacct tttctaaatc
      541 cttgatacca tcagtctcta ctcctccact tacatcgaca ccgatcgcat ttggtaacat
      601 attaatggca acagaaacgt tatcaggatt caatccacca gcgataatga attttatctc
      661 gggatgactt gcagaccaac tggaaattgc actccaattc aatttctcac cagtgccacc
      721 ttcaccagaa tcgaacaacg tcagcacatt gtctacgtgt tcatacaggt ccagtagtaa
      781 ttcacaatcc tgtgggaact ggaacctctt aatgattgga attgaagatg ggatcaaaga
      841 tctgtattct ttaatatctt catctccatg taattgtatc acatctagat tatattcgtg
      901 gtacagttga aggacatcat caacggactg atttctaaa
//
