LOCUS       sequence-95366-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: TFF1_pCSdest
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(320..344)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(398..416)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(399..416)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaaaatt cacactcctc ttctggaggg acgtcgatgg tattaggata gaagcaccag
      121 gggaccccac gaacggtgtc gtcgaaacag cagcccttat ttgcacactg ggagggcgtg
      181 acaccaggaa aaccacaatt ctgtctttca cggggggcca ctgtacacgt ctctgtctgg
      241 gcctcggcca gggtgccgag ggccagcatg gacaccagga ccagggcgca gatcaccttg
      301 ttctccatgg tggccatggt gcctgctttt ttgtacaaac ttgtgatcct tgaattcgaa
      361 tcgatgggat cctgcaaaaa gaacaagtag cttgtattct atagtgtcac ctaaatcaag
      421 cttgctccga cgtccccagg cagaatggcg gttccctaaa cgagcattgc ttatatagac
      481 ctcccattag gcacgcctac cgcccattta cgtcaatgga acgcccattt gcgtcattgc
      541 ccctccccat tgacgtcaat ggggatgtac ttgacagcca tcgcgggcca tttaccgcca
      601 ttgacgtcaa tgggagtact gccaatgtac cctg
//
