LOCUS       sequence-95376-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras with a G12D Mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras G12D
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..48
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             join(70..102,103..105,106..636)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             70..636
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(648..668)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atcaatggct tcaagtttcc atatgatgtg cccgactatg cctcacttgg aggaccctct
       61 ggatccacca tgactgaata taaacttgtg gtagttggag ctgacggcgt aggcaagagt
      121 gccttgacga tacagctaat tcagaatcat tttgtggacg aatatgatcc aacaatagag
      181 gattcctaca ggaagcaagt agtaattgat ggagaaacct gtctcttgga tattctcgac
      241 acagcaggtc aagaggagta cagtgcaatg agggaccagt acatgaggac tggggagggc
      301 tttctttgtg tatttgccat aaataatact aaatcatttg aagatattca ccattataga
      361 gaacaaatta aaagagttaa ggactctgaa gatgtaccta tggtcctagt aggaaataaa
      421 tgtgatttgc cttccagaac agtagacaca aaacaggctc aggacttagc aagaagttat
      481 ggaattcctt ttattgaaac atcagcaaag acaagacagg gtgttgatga tgccttctat
      541 acattagttc gagaaattcg aaaacataaa gaaaagatga gcaaagatgg taaaaagaag
      601 aaaaagaagt caaagacaaa gtgtgtaatt atgtaagtcg accctgtgga atgtgtgtca
      661 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      721 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      781 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttt
//
