LOCUS       sequence-95386-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PLAC4_pCSdest
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(479..503)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(557..575)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(558..575)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taatacgact cactatagtt ctagaggctc gagaggatca ccactttgta caagaaagct
       61 gggtcggcgc gcccaccctt tcaggactgg gggactgaag agactctggg gaggcttatg
      121 aactttctat ggggagcagc taggtgggga tcccttatgc atggcattct aatgcctgga
      181 agtaacgtga tccagtatag agccataaaa gcctgtacat aagggatctc ttcccatttt
      241 ccttctcttt tacaaaataa gcctaatcgt aaaatatcac tatagtatat agaaccatgt
      301 ttaggccaga tatattcgtc gtctaacttg tatttaaccc aaatggtgtt gcaatacaaa
      361 atgagtttct ttttctttaa gccaaatttg aatttgctcc aatagcttaa aagacaccct
      421 agcggcgagt cccttgggat actccttgtt gtccccatgg tgaagggggc ggccgcggag
      481 cctgcttttt tgtacaaact tgtgatcctt gaattcgaat cgatgggatc ctgcaaaaag
      541 aacaagtagc ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc
      601 agaatggcgg ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc
      661 gcccatttac gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg
      721 gggatgtact tggcagccat cgcgggccat ttaccgccat tgacgtcaat gggagtactg
      781 ccaatgtacc ctggcgtact tccaatagta atgtacttgc caagttacta
//
